Substantial sequence differentiation of three cpDNA genes between cryptic species within Conocephalum conicum /salebrosum complex.

Hanna Kijak, Aleksandra Spychała, Ireneusz Odrzykoski  
A. Mickiewicz University, Faculty of Biology, Poznań, PL-61614, Poland,

The liverwort genus Conocephalum Hill is composed of three morphospecies: C. conicum L. (Dum), recently described C. salebrosum Szweyk. Buczk. Odrzyk. and  C. japonicum (Thunb.) Grolle.  It was postulated that both C. conicum and C. japonicum include four and three cryptic species respectively, with more restricted allopatric distribution. Originally, genetic differentiation was studied using isozyme markers (Odrzykoski, Szweykowski 1991, Akiyama, Hiraoka 1994, Miwa 2004). The aim of this study was to analyze the sequence differentiation of large fragments of three chloroplast genes: rbcL, matK, psbA (and additionally trnK-psbA intergenic spacer) to examine whether studied sequences contain mutations, which can be used in the identification of cryptic species. Samples used in this study represent all known cryptic species supplemented by sequences from two publications (Kim et al. 2001, Miwa et al, 2009).

All analyzed sequences shown the presence of species specific substitutions. Within the intergenic region trnK- psbA we have also detected indels. The differences between cryptic species ranged between 0.001 to 0.011 substitutions per site. Most of the observed substitutions (70%) are synonymous. Intraspecific differentiation within cryptic species is very low except C. salebrosum sampled from the whole Holarctic range. We conclude that diagnostic mutations within psbA and two DNA barcode genes (rbcL, matK), are useful as species specific molecular markers within the Conocephalum conicum/salebrosum complex.

(This study has been supported by the NCN Grant no. N303 800340.)

The complex thalloids (Marchantiophyta) in Cuba: challenges and perspectives 

Yoira Rivera Queralta

Centro Oriental de Ecosistemas y Biodiversidad (BIOECO). Ministerio de Ciencia, Tecnología y Medio Ambiente (CITMA).  Santiago de Cuba, Cuba.

The main challenge of the botanists in Cuba is to upgrade bryological studies of the island. The present work aims at revising the state of the knowledge of the complex thalloids (Marchantiopsida) of Cuba. The Cuban species are listed and some aspects of their distribution and conservation are evaluated.

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